Reaction
Kinetics
Both
single cell and multi
cell Kinetics are available
with reaction plots to
screen and printer. Regression
analysis is applied to
the user-selected portion
of reaction curves and
results are reported in
Units/Litre. Data may be
reprocessed as required
and curves stored. Sample
temperature
control is available for
single cells and four and six
cell changers.
Advanced
Kinetics
An
example of advanced
kinetics is shown below where
time based reaction data
generated by
the spectrophotometer has
been transferred into Grafit,
fitted
to a curve and transformed
into a more suitable format
for mathematical interpolation
and calculation. In this
particular
example, the basic data
plot can yield the limiting
rate (Vmax), the Michaelis
constant
(Km) and the initial
linear portion (Vmax/Km).
This
has been transformed into
the Eadie-Hofstee format
and plotted to give better
precision
in the calculation of
these important factors and
constants.
Other transformations
are available such
as Lineweaver-Burk and
the Hill plot for allosteric
enzyme systems.
PC
Data Manipulation
Data
may be transferred to
a PC for use with spread
sheets and other mathematical
software packages. More advanced
reaction kinetics data manipulation
may be readily performed
with
Grafit software. Linear,
non-linear or polynomial
curve fitting
is easily accomplished together
with non-linear regression
analysis. A wide selection
of standard
equations are provided, together
with an equation editor for
customised equation fitting.
Data may also
be transformed into other
mathematical formats
for plotting and calculation.
Band
Quantification
Accurate
quantitative assays
using band area or height,
corrected for background
absorption,
are exceptionally easy
to carry out with the easy
to use Aquarius instruments.
Analytical
results are displayed and
printed with construction
lines enabling the quality
of the parameters to be
assessed.
The procedure
may be stored
as
a method with security
code protection in the
integral memory of the instrument.
